Structure of IDP91538

EutD phosphotransacetylase from Staphylococcus aureus.

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CSGID target
IDP91538 
PDB Id
4E4R (NCBI MMDB) 
Authors
J.Osipiuk,M.Zhou,S.Peterson,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Mar 13, 2012 
Release Date
Mar 28, 2012 

Annotation

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Description
EutD phosphotransacetylase converts acetyl coenzyme A and phosphate into coenzyme A and acetyl phosphate. The enzyme is a part of ethanolamine utilization (eut) system allowing bacteria to use ethanolamine as a source of carbon, nitrogen and energy. The EutD phosphotransacetylase displays nearly the same biological activity as the housekeeping phosphotransacetylases, Pta enzymes.  
Functional assignment
transferase 

Ligands

Ligand code Name Ligand type
EDO ethylene diol crystallization
TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL crystallization
MSE modified residue
175 3,5-dihydro-5-methylidene-4h-imidazol-4-on

Structure information

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Unit cell parameters

Space Group
P 41 21 2  
Unit Cell

a=54.59Å, b=54.59Å, c=202.90Å
α=90.00, β=90.00, γ=90.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
42.50-1.44Å (1.48-1.44Å)  
Rall(%)
13.0 
Rwork(%)
12.8 (22.6) 
Rfree(%)
18.0 (29.1) 
Num. observed reflections
56826 (3688) 
Num. Rfree reflections
2898 (175) 
Completeness(%)
99.8 (97.8) 

Model parameters

Num Atoms
2988  
Num Waters
317  
Num Hetatoms
0  
Model mean isotropic B factor
20.250Å2  
RMSD bond length
0.014Å  
RMSD bond angle
1.896°  
Filename uploaded
idp91538.pdb (uploaded on Mar 13, 2012 12:54 PM)  
Inserted
Mar 13, 2012