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Structure of IDP01325

CRYSTAL STRUCTURE OF A PUTATIVE ORGANIC HYDROPEROXIDE RESISTANCE PROTEIN FROM VIBRIO CHOLERAE O1BIOVAR ELTOR STR. N16961

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CSGID target
IDP01325 
PDB Id
3I07 (NCBI MMDB
Authors
'B.Nocek,N.Maltseva,K.Kwon,W.Anderson,A.Joachimiak' 
Responsible person
Boguslaw Nocek 
Responsible lab
Argonne National Laboratory 
Deposition Date
Jun 24, 2009 
Release Date
Aug 11, 2009 

Annotation

Description
The organic hydroperoxide resistance protein, thiol-dependent peroxidase that is a central player in response to stress induced by organic hydroperoxides in bacteria. It has unique three-dimensional structure and requires dithiols to support its activity. It forms an oval-shaped, tight homodimer and have two active site positioned on the opposite faces of the dimer. We have determined series of the structure of OHR-like protein from V. cholera and were able to reveal the enzymes structure in the reduce state (3I07), in the presence of captopril molecule (3LUS). FOX assays confirmed that it metabolizes hydrogen peroxide. 
Functional assignment
OHR 

Ligands

Ligand code Name Ligand type
GOL glycerol
MES MES

Structure information

Unit cell parameters

Space Group
P 21 21 21  
Unit Cell

a=38.03Å, b=76.15Å, c=79.69Å
α=90.00, β=90.00, γ=90.00 
Solvent content
34.58  
Matthews coefficient
1.88  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
20.00-1.50Å (1.54-1.50Å)  
Rall(%)
17.0 
Rwork(%)
16.5 (23.1) 
Rfree(%)
18.5 (28.2) 
Num. observed reflections
35796 (1800) 
Num. Rfree reflections
1789 (104) 
Completeness(%)
95.1 (65.9) 

Model parameters

Num Atoms
2180  
Num Waters
275  
Num Hetatoms
299  
Model mean isotropic B factor
7.340Å2  
RMSD bond length
0.012Å  
RMSD bond angle
1.425°  
Filename uploaded
3I07.pdb (uploaded on Sep 16, 2009 4:59 PM)  
Inserted
Jul 02, 2009