Center for Structural Genomics of Infectious Diseases

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Structure of IDP02311

CSGID target: IDP02311
PDB Id: 3MJD (NCBI MMDB)
Authors: G.Minasov,L.Shuvalova,I.Dubrovska,J.Winsor,L.Papazisi,W.F.Anderson,Center For Structural Genomics Of Infectious Diseases (Csgid)
Responsible person: George Minasov
Responsible lab: Northwestern University
Deposition Date: Apr 12, 2010
Release Date: Apr 21, 2010

Description: Orotate phosphoribosyltransferase (OPRTase) is involved in the biosynthesis of pyrimidine nucleotides. Alpha-D-ribosyldiphosphate 5-phosphate (PRPP) and orotate are utilized to form pyrophosphate and orotidine 5'-monophosphate (OMP) in the presence of divalent cations, preferably Mg2+. OMP is thereafter converted to uridine 5'-monophosphate by OMP decarboxylase. We have determined the 1.9 angstrom structure of Francisella tularensis subsp. tularensis SCHU S4 OPRTase (PyrE). The OPRTase three-dimensional structure reveals two major features: a core five-stranded alpha/beta twisted sheet and an N-terminal region that partially covers the C-terminal portion of the core. PRTases show a very high degree of base specificity.
Functional assignment: Purines, pyrimidines, nucleosides, and nucleotides; subcategory: Pyrimidine ribonucleotide biosynthesis

Ligand code	Name	Ligand type
TRS	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	crystallization
175	3,5-dihydro-5-methylidene-4h-imidazol-4-on