Center for Structural Genomics of Infectious Diseases - Deposits

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Structure of IDP90718

Putative RNA methyltransferase from Yersinia pestis.

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CSGID target: IDP90718
PDB Id: 3N4J (NCBI MMDB)
Authors: 'J.Osipiuk,N.Maltseva,S.Peterson,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)'
Responsible person: Jerzy Osipiuk
Responsible lab: Argonne National Laboratory
Deposition Date: May 21, 2010
Release Date: Jun 23, 2010

Annotation

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Description: Putative RNA methyltransferase (MTase) from Yersinia pestis is similar to Escherichia coli hypothetical tRNA/rRNA methyltransferase YibK and Haemophilus influenzae YibK methyltransferase. These enzymes belong to the SpoU family of MTases, a subfamily of the α/β-knot superfamily. A putative reaction of the Yersinia pestis enzyme is 2’-O-methylation of leucyl tRNA isoacceptors.
Functional assignment: Transferase

Ligands

Ligand code	Name	Ligand type
SO4	sulfate

Structure information

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Unit cell parameters

Space Group: P 31 2 1
Unit Cell: a=79.72Å, b=79.72Å, c=44.64Å
α=90.00, β=90.00, γ=120.00
Solvent content: 44.34
Matthews coefficient: 2.21
Resolution range: 34.60-1.47Å (1.51-1.47Å)
R_all(%): 14.5
R_work(%): 14.3 (22.8)
R_free(%): 18.6 (29.3)
Num. observed reflections: 26316 (1348)
Num. R_free reflections: 1342 (71)
Completeness(%): 94.1 (66.6)
Num Atoms: 1489
Num Waters: 144
Num Hetatoms: 0
Model mean isotropic B factor: 17.120Å²
RMSD bond length: 0.019Å
RMSD bond angle: 1.670°
Filename uploaded: idp90718.pdb (uploaded on May 21, 2010 6:47 PM)
Inserted: May 21, 2010