Annotation

Description

ABC transporters utilize the energy of ATP hydrolysis to transport various substrates across cellular membranes. In prokaryotes, importers mediate the uptake of nutrients into the cell. The substrates that can be transported include ions, amino acids, peptides, sugars, and other molecules that are mostly hydrophilic. The membrane-spanning region of the ABC transporter protects hydrophilic substrates from the lipids of the membrane bilayer thus providing a pathway across the cell membrane. Transporters are extremely vital in cell survival such that they function as protein systems that counteract any undesirable change occurring in the cell. For instance, a potential lethal increase in osmotic strength is counterbalanced by activation of osmosensing ABC transporters that mediate uptake of solutes. Other than functioning in transport, some bacterial ABC proteins are also involved in the regulation of several physiological processes. ABC transporters are known to play a crucial role in the development of multidrug resistance. 

Functional assignment

 

Structure information

Unit cell parameters

Space Group

P 21 21 21  

Unit Cell

a=61.94Å, b=66.76Å, c=115.49Å
α=90.00, β=90.00, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

29.91-1.50Å (1.54-1.50Å)  

R_all(%)

14.3 

R_work(%)

14.1 (18.7) 

R_free(%)

17.0 (20.6) 

Num. observed reflections

77232 (5601) 

Num. R_free reflections

3861 (299) 

Completeness(%)

99.8 (99.6) 

Model parameters

Num Atoms

4044  

Num Waters

736  

Num Hetatoms

903  

Model mean isotropic B factor

15.240Ų  

RMSD bond length

0.012Å  

RMSD bond angle

1.588°  

Filename uploaded

3RQT.pdb (uploaded on May 25, 2011 12:40 PM)  

Inserted

May 25, 2011