Clone

Target

IDP90523  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

284  

Reference Sequence Used

MLIIETLPLL RQQIRRWRQE GKRIALVPTM GNLHEGHMTL VDEAKTRADV VVVTIFVNPL QFERPDDLAH YPRTLQEDCE KLTRHGADLV FAPAAADIYP AGLEKQTYVD VPALSTILEG ASRPGHFRGV STIVSKLFNL IQPDVACFGE KDYQQLALIR KMVADMGYDI NIVGVPTVRA KDGLALSSRN GYLTEEERQI APQLSKIMWA LAEKMALGER QIDALLEEAA AQLLRVGFTP DELFIRDAET LQPLTVDSQQ AVILMAAWLG KARLIDNQLV DLRH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLIIETLPLL RQQIRRWRQE GKRIALVPTM GNLHEGHMTL VDEAKTRADV VVVTIFVNPL QFERPDDLAH YPRTLQEDCE KLTRHGADLV FAPAAADIYP AGLEKQTYVD VPALSTILEG ASRPGHFRGV STIVSKLFNL IQPDVACFGE KDYQQLALIR KMVADMGYDI NIVGVPTVRA KDGLALSSRN GYLTEEERQI APQLSKIMWA LAEKMALGER QIDALLEEAA AQLLRVGFTP DELFIRDAET LQPLTVDSQQ AVILMAAWLG KARLIDNQLV DLRH  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTGATTATTGAGACATTGC  

PCR Backward Primer

TTATCCACTTCCAATGTCAGTGGCGCAAATCAACCA  

Shipped To Lab

ANL  

Shipped Date

Nov 03, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009