Clone

Target

IDP90687  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Oct 28, 2009  

Experiment End Date

Oct 28, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

354  

Reference Sequence Used

MGVLDIVKAG VISGDELNKI YDYAKAEGFA IPAVNVVGTD SINAVLEAAK KVNSPVIIQF SNGGAKFYAG KNCPNGEVLG AISGAKHVHL LAKAYGVPVI LHTDHAARKL LPWIDGLIEA NAQYKKTHGQ ALFSSHMLDL SEESLEENLS TCEVYLQKLD ALGVALEIEL GCTGGEEDGV DNTGIDNSKL YTQPEDVALA YERLGKISDK FSIAASFGNV HGVYKPGNVS LQPEILKNSQ KFVKDKFALN SDKPINFVFH GGSGSELKDI KNAVSYGVIK MNIDTDTQWA FWDGVREYEL KNRAYLQGQI GNPEGDDKPN KKYYDPRVWL RSGEESMIKR LEIAFEDLNC INKN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MGVLDIVKAG VISGDELNKI YDYAKAEGFA IPAVNVVGTD SINAVLEAAK KVNSPVIIQF SNGGAKFYAG KNCPNGEVLG AISGAKHVHL LAKAYGVPVI LHTDHAARKL LPWIDGLIEA NAQYKKTHGQ ALFSSHMLDL SEESLEENLS TCEVYLQKLD ALGVALEIEL GCTGGEEDGV DNTGIDNSKL YTQPEDVALA YERLGKISDK FSIAASFGNV HGVYKPGNVS LQPEILKNSQ KFVKDKFALN SDKPINFVFH GGSGSELKDI KNAVSYGVIK MNIDTDTQWA FWDGVREYEL KNRAYLQGQI GNPEGDDKPN KKYYDPRVWL RSGEESMIKR LEIAFEDLNC INKN  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGTGTTTTAGACATTGTAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTTTTGTTTATACAGTTTAAAT  

Shipped To Lab

UT  

Shipped Date

May 03, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 04, 2009