Clone

Target

IDP04396  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

331  

Reference Sequence Used

MKVAENQKVY DITIIGGGPT GLFTAFYGGM RQASVKIIES LPQLGGQLSA LYPEKYIYDV AGFPKVRAQE LVDNLKEQMK KFDPTVCLEE AVDTLEKQAD GIFKLVTNKQ THYSKSVIIT AGNGAFQPRR LELEGTAKYE KKNLHYFVDD MNKFAGKRVV VFGGGDSAVD WTMMLEPIAD KVTIVHRRDK FRAHEHSVES LMNSRAEVST PYVPVELIGD DKIEQVVLQH VKTEEKIIID VDDVIVNYGF VSSLGPIKNW GLDIQKNSIL VNSKMETNIP GIYAAGDICT YEGKVKLIAC GFGEAPTAVN NAKAYFDPNA KLQPMHSSSM F  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKVAENQKVY DITIIGGGPT GLFTAFYGGM RQASVKIIES LPQLGGQLSA LYPEKYIYDV AGFPKVRAQE LVDNLKEQMK KFDPTVCLEE AVDTLEKQAD GIFKLVTNKQ THYSKSVIIT AGNGAFQPRR LELEGTAKYE KKNLHYFVDD MNKFAGKRVV VFGGGDSAVD WTMMLEPIAD KVTIVHRRDK FRAHEHSVES LMNSRAEVST PYVPVELIGD DKIEQVVLQH VKTEEKIIID VDDVIVNYGF VSSLGPIKNW GLDIQKNSIL VNSKMETNIP GIYAAGDICT YEGKVKLIAC GFGEAPTAVN NAKAYFDPNA KLQPMHSSSM F  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGTGGCAGAAAATCAAAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAACATACTAGAGCTGTGC  

Shipped To Lab

ANL  

Shipped Date

Dec 29, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009