Purification

Target

IDP00499  

Purification ID

A4943  

Expression

M9 minimal (Oct 15, 2008)  

Experiment Date Start

Jan 22, 2009  

Experiment Date End

Jan 24, 2009  

Person

Rory Mulligan  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

37  

Final Volume (μL)

1200  

Shipped To Lab Id

ANL  

Shipped Date

Jan 24, 2009  

Inserted

Jul 29, 2011