Purification

Target
IDP00038  
Purification ID
A6440  
Expression
M9 minimal + semet (Feb 28, 2011)  
Experiment Date Start
Feb 28, 2011  
Experiment Date End
Mar 02, 2011  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
77  
Final Volume (μL)
600  
Shipped To Lab Id
ANL  
Shipped Date
Mar 02, 2011  
Inserted
Jul 29, 2011