Annotation

Description

Transaldolase B is an enzyme of the Class I aldose family and catalyzes the reversible transer of a dihydroxyacetone moiety, derived from fructose-6-phosphate, to erythrose-4-phosphate yielding sedohepptulose-7-phosphate. This reaction is a part of the pentose phosphate pathway, an anabolic pathway that utilizes the 6 carbons of glucose to generate 5 carbon sugars and reducing equivalents. A common feature of the members of this enzyme family is the formation of a covalent intermediate (Schiff base) between an active site lysine residue and the substrate during catalysis. The enzyme is of considerable interest as a catalyst in stereospecific organic synthesis. It is also a target for drug design, because Transaldolase (TAL) is involved in oxidative stress and apoptosis, in multiple sclerosis, and its deficiency leads to severe symptoms in human.  

Functional assignment

Transaldolase B 

Structure information

Unit cell parameters

Space Group

P 21 21 21  

Unit Cell

a=56.49Å, b=75.86Å, c=164.77Å
α=90.00, β=90.00, γ=90.00 

Solvent content

49.43  

Matthews coefficient

2.43  

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

29.41-1.85Å (1.90-1.85Å)  

R_all(%)

15.1 

R_work(%)

15.0 (20.4) 

R_free(%)

18.6 (26.2) 

Num. observed reflections

61062 (4285) 

Num. R_free reflections

3114 (212) 

Completeness(%)

99.6 (96.0) 

Model parameters

Num Atoms

5142  

Num Waters

847  

Num Hetatoms

979  

Model mean isotropic B factor

12.650Ų  

RMSD bond length

0.011Å  

RMSD bond angle

1.290°  

Filename uploaded

validate-2.pdb (uploaded on Jul 29, 2009 11:44 AM)  

Inserted

Jul 29, 2009