Center for Structural Genomics of Infectious Diseases

Expression

Target: IDP01610
Expression ID: A1393
Protein Clone: pMCSG7 (Jan 28, 2009)
Experiment Date Start: Mar 02, 2009
Experiment Date End: Mar 04, 2009
Person: Min Zhou
Lab: Argonne National Laboratory
Protocol: standard expression_ANL
Status: success
Expression Organism: E. coli
Expression Strain: BL21(DE3)magic
Media Type: M9 minimal + semet
Media Volume: 2000 mL
Growth Temperature: 37 °C
Induction Temperature: 16 °C
Induction Reagent: 1 mM IPTG
Experiment Type: production
Expression Level: 100.0 %
Solubility Level: 100.0 %
Inserted: Jul 29, 2011

Related Purifications

Experiment Start Date	Experiment End Date	Person	Lab	Protocol	Status	Affinity Tag Status	SeMet Status	Final Buffer	Shipped To Lab	Shipped Date
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06
Mar 04, 2009	Mar 06, 2009	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	cleaved	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2009-03-06