Expression

Target
IDP52003  
Expression ID
A12045  
Protein Clone
pMCSG53 (Apr 01, 2020)  
Experiment Date Start
Oct 14, 2020  
Experiment Date End
Oct 16, 2020  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-Gold(DE3)  
Media Type
LB + 40 mM K2HPO4  
Media Volume
8000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
80.0 %  
Solubility Level
50.0 %  
Inserted
Aug 23, 2021  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date