Clone

Target

IDP01484  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

394  

Reference Sequence Used

MLTQEHINII KSTIPLLESA GPALTQHFYQ RMFSHNPELK HIFNMTHQKT GRQSVALFEA IAAYAKHIDN LAALTSAVER IAHKHTSFNI QPEHYQIVGH HLLETLRELA PDAFTQPVEE AWTAAYFFLA QVFIDREGAL YLERKQALGG WRDGRTFVVR EKQVESAYVT SFVLVPADGG AVLDYQPGQY IGIEVTPEGS DYREIRQYSL SHASNGREYR ISVKREGVGS DNPGLVSHYL HNNVKVGDSV KLYAPAGDFF YVERERPVVL ISAGVGATPM QAILHTLAKQ NKSGVTYLYA CNSAKEHTFA QETAQLIAQQ GWMQQVWYRD ESADDVLQGE MQLAELILPI EDGDFYLCGP IGFMQYVVKQ LLALGVDKAR IHYEVFGPHA QLAA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MLTQEHINII KSTIPLLESA GPALTQHFYQ RMFSHNPELK HIFNMTHQKT GRQSVALFEA IAAYAKHIDN LAALTSAVER IAHKHTSFNI QPEHYQIVGH HLLETLRELA PDAFTQPVEE AWTAAYFFLA QVFIDREGAL YLERKQALGG WRDGRTFVVR EKQVESAYVT SFVLVPADGG AVLDYQPGQY IGIEVTPEGS DYREIRQYSL SHASNGREYR ISVKREGVGS DNPGLVSHYL HNNVKVGDSV KLYAPAGDFF YVERERPVVL ISAGVGATPM QAILHTLAKQ NKSGVTYLYA CNSAKEHTFA QETAQLIAQQ GWMQQVWYRD ESADDVLQGE MQLAELILPI EDGDFYLCGP IGFMQYVVKQ LLALGVDKAR IHYEVFGPHA QLAA  

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTCACCCAAGAACACATC  

PCR Backward Primer

TTATCCACTTCCAATGTTACGCCGCAAGCTGTGCA  

Shipped To Lab

ANL  

Shipped Date

Oct 09, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008