Clone

Target

IDP01970  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Jan 28, 2009  

Experiment End Date

Jan 29, 2009  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

181  

Reference Sequence Used

MTEEHVVLLD EQDKPSGTLE KYAAHTLNTP LHLAFSCWLF NEDGQLLVTR RSLSKKAWPG VWTNSVCGHP QQGETTEEAI IRRCRFELGV EITDLTPVYP HFSYRATDPN GIVENEVCPV FAARATSVLQ VNSEEVMDYQ WSEFKSVWKS LLATPWAFSP WMVMQASDEQ ARERLLNYCQ R  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MTEEHVVLLD EQDKPSGTLE KYAAHTLNTP LHLAFSCWLF NEDGQLLVTR RSLSKKAWPG VWTNSVCGHP QQGETTEEAI IRRCRFELGV EITDLTPVYP HFSYRATDPN GIVENEVCPV FAARATSVLQ VNSEEVMDYQ WSEFKSVWKS LLATPWAFSP WMVMQASDEQ ARERLLNYCQ R  

PCR Forward Primer

TACTTCCAATCCAATGCGATGACTGAGGAACATGTCGTT  

PCR Backward Primer

TTATCCACTTCCAATGTTAACGCTGGCAGTAATTGAG  

Shipped To Lab

ANL  

Shipped Date

Feb 19, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Feb 24, 2009