Clone

Target

IDP00085  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 20, 2009  

Experiment End Date

Apr 20, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

341  

Reference Sequence Used

MGPVMLNVEG CELDAEEREI LAHPLVGGLI LFTRNYHDPE QLRELVRQIR AASRNHLVVA VDQEGGRVQR FREGFTRLPA AQSFFALHGL EEGGRLAQEA GWLMASEMIA MDIDISFAPV LDVGHISAAI GERSYHADPA KALAMATRFI DGMHDAGMKT TGKHFPGHGA VTADSHKETP CDPRPETDIR GKDMSVFRTL ISENKLDAIM PAHVIYRAID PRPASGSPYW LKTVLRQELG FDGVIFSDDL SMEGAAIMGS YAERAQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV TRLYHKGSFS RRELMDSARW KTASAQLNQL HERWQEEKAG H  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MGPVMLNVEG CELDAEEREI LAHPLVGGLI LFTRNYHDPE QLRELVRQIR AASRNHLVVA VDQEGGRVQR FREGFTRLPA AQSFFALHGL EEGGRLAQEA GWLMASEMIA MDIDISFAPV LDVGHISAAI GERSYHADPA KALAMATRFI DGMHDAGMKT TGKHFPGHGA VTADSHKETP CDPRPETDIR GKDMSVFRTL ISENKLDAIM PAHVIYRAID PRPASGSPYW LKTVLRQELG FDGVIFSDDL SMEGAAIMGS YAERAQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV TRLYHKGSFS RRELMDSARW KTASAQLNQL HERWQEEKAG H  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGCCCAGTAATGTTGAAT  

PCR Backward Primer

TTATCCACTTCCAATGTTAATGACCTGCTTTTTCTTCC  

Shipped To Lab

UT  

Shipped Date

Apr 30, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 21, 2009