Clone

Target

IDP01739  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

384  

Reference Sequence Used

MSEILVLNCG SSSVKFALIN PHTSQSLVTG LAENIATKNC KVVFKAEHKI VKYLENGSYK DVFEMLKDFL VENKHLEKIV AIGHRVVHGG QYFSKSVLIN ADSLEKIKAC IALAPLHNPA HIEGIRFCQQ IFPELPQVAV FDTAFHQTMP SYIAEYAIPY ELTHKHNIRK YGAHGTSHKY VSEQAAKILT QQKANVIVAH LGNGCSITAV VDGKSIDTSM GLTPLDGLVM GTRSGCIDPS IFAYISDNLG WSVTEITNML NKQSGLLGIC GHNDMREVSQ LAAKGDSLAK LAIEIFSHRV AKFVASYMIY FNKLDALVFT GGIGENAANI RKNIISKLAN LGFMIDHQKN SNSETFINSK NSHNIMVIAT NEELMIAQET QNLI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSEILVLNCG SSSVKFALIN PHTSQSLVTG LAENIATKNC KVVFKAEHKI VKYLENGSYK DVFEMLKDFL VENKHLEKIV AIGHRVVHGG QYFSKSVLIN ADSLEKIKAC IALAPLHNPA HIEGIRFCQQ IFPELPQVAV FDTAFHQTMP SYIAEYAIPY ELTHKHNIRK YGAHGTSHKY VSEQAAKILT QQKANVIVAH LGNGCSITAV VDGKSIDTSM GLTPLDGLVM GTRSGCIDPS IFAYISDNLG WSVTEITNML NKQSGLLGIC GHNDMREVSQ LAAKGDSLAK LAIEIFSHRV AKFVASYMIY FNKLDALVFT GGIGENAANI RKNIISKLAN LGFMIDHQKN SNSETFINSK NSHNIMVIAT NEELMIAQET QNLI  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCTGAAATATTAGTATTAAATTG  

PCR Backward Primer

TTATCCACTTCCAATGTTATATTAAATTTTGTGTTTCTTGAG  

Shipped To Lab

UT  

Shipped Date

May 14, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009