Clone

Target

IDP02119  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 27, 2009  

Experiment End Date

Apr 29, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

224  

Reference Sequence Used

MFFNKKNNQD ELKKLAATEA AKSITTEITL GVGTGSTVGF LIEELVNYRD KIKTVVSSSE DSTRKLKALG FDVVDLNYAG EIDLYIDGAD ECNNHKELIK GGGAALTREK ICVAAAKKFI CIIDESKKVN TLGNFPLPIE VIPMARSYIA RQIVKLGGQP VYREQTITDN GNVILDVYNL KIDNPLKLET ELNQITGVVT NGIFALKPAD TVIMATKDSN IVVL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MFFNKKNNQD ELKKLAATEA AKSITTEITL GVGTGSTVGF LIEELVNYRD KIKTVVSSSE DSTRKLKALG FDVVDLNYAG EIDLYIDGAD ECNNHKELIK GGGAALTREK ICVAAAKKFI CIIDESKKVN TLGNFPLPIE VIPMARSYIA RQIVKLGGQP VYREQTITDN GNVILDVYNL KIDNPLKLET ELNQITGVVT NGIFALKPAD TVIMATKDSN IVVL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTTTTAATAAAAAAAATAACCAA  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAGTACAACAATATTACTATCT  

Shipped To Lab

UTSW  

Shipped Date

May 14, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 05, 2009