Clone

Target

IDP00252  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

408  

Reference Sequence Used

MFNCLGTSNR DFVEGASGAT WIDLVLEGGS CVTVMAPEKP TLDFKVMKME ATELATVRKY CYEATLDTLS TVARCPTTGE AHNTKRSDPT FVCKRDVVDR GWGNGCGLFG KGSIDTCAKF TCKNKATGKT ILRENIKYEV AIFVHGSTDS TSHGNYSEQI GKNQAARFTI SPQAPSFTAN MGEYGTVTID CEARSGINTE DYYVFTVKEK SWLVNRDWFH DLNLPWTSPA TTDWRNRETL VEFEEPHATK QTVVALGSQE GALHTALAGA IPATVSSSTL TLQSGHLKCR AKLDKVKIKG TTYGMCDSAF TFSKNPTDTG HGTVIVELQY TGSNGPCRVP ISVTANLMDL TPVGRLVTVN PFISTGGANN KVMIEVEPPF GDSYIVVGRG TTQINYHWHK EGSSIGKAL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTCAACTGTTTGGGAACAT  

PCR Backward Primer

TTATCCACTTCCAATGCAAAGCCTTCCCAATGCTGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009