Clone

Target

IDP00254  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

407  

Reference Sequence Used

MFNCLGMGNR DFIEGASGAT WVDLVLEGDS CLTIMANDKP TLDVRMINIE ASQLAEVRSY CYHASVTDIS TVARCPTTGE AHNEKRADSS YVCKQGFTDR GWGNGCGLFG KGSIDTCAKF SCTSKAIGRT IQPENIKYKV GIFVHGTTTS ENHGNYSAQV GASQAAKFTV TPNAPSITLK LGDYGEVTLD CEPRSGLNTE AFYVMTVGSR SFLVHREWFH DLALPWTSPS STAWRNRELL MEFEEAHATK QSVVALGSQE GGLHQALAGA IVVEYSSSVK LTSGHLKCRL KMDKLALKGT TYGMCTEKFS FAKNPADTGH GTVVIELSYS GSDGPCKIPI VSVASLNDMT PVGRLVTVNP FVATSSANSK VLVEMEPPFG DSYIVVGRGG KQINHHWHKA GSTLGKAF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTAATTGTCTGGGAATGG  

PCR Backward Primer

TTATCCACTTCCAATGAAAGGCTTTGCCCAGCGTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009