Clone

Target

IDP00272  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2009  

Experiment End Date

Jul 24, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

496  

Reference Sequence Used

MRCVGIGNRD FVEGLSGATW VDVVLEHGSC VTTMAKNKPT LDIELLKTEV TNPAVLRKLC IEAKISNTTT DSRCPTQGEA TLVEEQDANF VCRRTFVDRG WGNGCGLFGK GSLITCAKFK CVTKLEGKIV QYENLKYSVI VTVHTGDQHQ VGNETTEHGT TATITPQAPT SEIQLTDYGT LTLDCSPRTG LDFNEMVLLT MKERSWLVHK QWFLDLPLPW TSGASTSQET WNRQDLLVTF KTAHAKKQEV VVLGSQEGAM HTALTGATEI QTSGTTTIFA GHLKCRLKMD KLTLKGMSYV MCTGSFKLEK EVAETQHGTV LVQVKYEGTD APCKIPFSTQ DEKGATQNGR LITANPIVTD KEKPVNIEAE PPFGESYIVV GAGEKALKLS WFKKGSSIGK MFEATARGAR RMAILGDTAW DFGSIGGVFT SMGKLVHQVF GTAYGVLFSG VSWTMKIGIG ILLTWLGLNS RNTSLSMMCI AVGMVTLYLG VMVQAD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGATGCGTGGGAATAGG  

PCR Backward Primer

TTATCCACTTCCAATGATCTGCCTGAACCATGACTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 10, 2009