Clone

Target

IDP05773  

Clone Suffix

1

Sequence Comments

Removed signal peptide (MMKMTMKMMVRIYFVSLSLSLSLLLFHSYA). Added Met at N-terminus.  

Experiment Start Date

Feb 01, 2009  

Experiment End Date

Feb 02, 2009  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pET23b  

Sequence Start

31  

Sequence End

538  

Reference Sequence Used

MIDIENEITE FFNKMRDTLP AKDSKWL NPSCM FGGTMNDMAA LGEPFSAKCP PIEDSLLSHR YNDKDNVVNW EKIGKTRRPL NRRVKNGDLW I A NYTSNDSHRR YLCTVTTKNG DCVQGIVRSH IRKPPSCIPE TYELGTHDKY GIDLYCGILY AKHYN NITWYKN NQELIIDGTK YSQSGQNLII HNPELEDSGR YDCYVHYDDV RIKNDIVVSR CKILTVIPS QDH RFKLILDPKI NVTIGEPANI TCTAVSTSLL VDDVLIDWEN PSGWIIGLDF GVYSILTSSG GIT EATLYFENV TEEYIGNTYT CRGHNYYFDK TLTTTVVLE  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

 

PCR Backward Primer

 

Shipped To Lab

WU  

Shipped Date

Feb 02, 2009  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 12, 2010