Clone

Target

IDP00557  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

145: S -> G  

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

481  

Reference Sequence Used

MKKTTLVLSA LALSIGFAMG PVSSVVAAET AASSSQQLPS LAPMLEKVMP SVVSINVEGS APVSSAGARG MPPQFQQFFG DNSPFCQDGS PFQGSPMCQG DLGGLGQGMP SKREFRSLGS GVIIDAGKGY VVTNNHVVDN ANKISVKLSD GRSFDAKVIG KDPRTDIALL QLKDAKNLTA IKIANSDQLR VGDYTVAIGN PYGLGETVTS GIVSALGRSG LNVENYENFI QTDAAINRGN SGGALINLNG ELIGINTAIL APDGGNIGIG FAIPSNMVKN LTSQMVEFGQ VKRGELGIMG TELNSELAKA MKVDAQKGAF ISQVVPKSAA AKAGIKAGDI IVSMNGKAIN SFAGFRAEIG TLPVGSKMTL GLLRDGKPIN VDVVLEQSSH SQVESGNLYT GIEGAELSNS DVSGKKGVKV DSVKPGTAAA RIGLKKGDII MGINQQPVQN LGELRKILDA KPPVLALNIQ RGDTSLYLLM Q  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKKTTLVLSA LALSIGFAMG PVSSVVAAET AASSSQQLPS LAPMLEKVMP SVVSINVEGS APVSSAGARG MPPQFQQFFG DNSPFCQDGS PFQGSPMCQG DLGGLGQGMP SKREFRSLGS GVIIDAGKGY VVTNNHVVDN ANKIGVKLSD GRSFDAKVIG KDPRTDIALL QLKDAKNLTA IKIANSDQLR VGDYTVAIGN PYGLGETVTS GIVSALGRSG LNVENYENFI QTDAAINRGN SGGALINLNG ELIGINTAIL APDGGNIGIG FAIPSNMVKN LTSQMVEFGQ VKRGELGIMG TELNSELAKA MKVDAQKGAF ISQVVPKSAA AKAGIKAGDI IVSMNGKAIN SFAGFRAEIG TLPVGSKMTL GLLRDGKPIN VDVVLEQSSH SQVESGNLYT GIEGAELSNS DVSGKKGVKV DSVKPGTAAA RIGLKKGDII MGINQQPVQN LGELRKILDA KPPVLALNIQ RGDTSLYLLM Q  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAAAAACAACTTTAGTATTAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACTGCATCAATAAATAGAGTG  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008