Purification

Target

IDP05622  

Purification ID

A10392  

Expression

M9 minimal (Jun 12, 2012)  

Experiment Date Start

Jun 20, 2012  

Experiment Date End

Jun 22, 2012  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

32  

Final Volume (μL)

200  

Shipped To Lab Id

ANL  

Shipped Date

Jun 27, 2012  

Inserted

Jul 06, 2012