Purification

Target

IDP90639  

Purification ID

A12202  

Expression

M9 minimal (Oct 29, 2012)  

Experiment Date Start

Nov 14, 2012  

Experiment Date End

Nov 20, 2012  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

183  

Final Volume (μL)

450  

Shipped To Lab Id

ANL  

Shipped Date

Nov 20, 2012  

Inserted

Nov 28, 2012