Purification

Target

IDP02260  

Purification ID

A16583  

Expression

M9 minimal + semet (Oct 07, 2013)  

Experiment Date Start

Jan 13, 2014  

Experiment Date End

Jan 14, 2014  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

21  

Final Volume (μL)

500  

Shipped To Lab Id

ANL  

Shipped Date

Jan 15, 2014  

Inserted

Jan 22, 2014