Purification

Target

IDP02549  

Purification ID

A16586  

Expression

M9 minimal (Oct 07, 2013)  

Experiment Date Start

Jan 13, 2014  

Experiment Date End

Jan 14, 2014  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

52  

Final Volume (μL)

1000  

Shipped To Lab Id

ANL  

Shipped Date

Jan 15, 2014  

Inserted

Jan 22, 2014