Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP02135
Purification ID: A17577
Expression: M9 minimal (Apr 14, 2014)
Experiment Date Start: May 19, 2014
Experiment Date End: May 21, 2014
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 26
Final Volume (μL): 25000
Shipped To Lab Id: ANL
Shipped Date: May 21, 2014
Inserted: May 30, 2014

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	36	CrystalQuick standard profile, LBR round bottom plate
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	22	CrystalQuick standard profile, LBR round bottom plate
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	91	CrystalQuick standard profile, LBR round bottom plate
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	91	CrystalQuick standard profile, LBR round bottom plate
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	69	CrystalQuick standard profile, LBR round bottom plate
May 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	68	CrystalQuick standard profile, LBR round bottom plate
Jun 10, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	2	CrystalQuick standard profile, LBR round bottom plate