Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP91646
Purification ID: A18381
Expression: M9 minimal (Aug 12, 2014)
Experiment Date Start: Sep 09, 2014
Experiment Date End: Sep 10, 2014
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 12
Final Volume (μL): 4
Shipped To Lab Id: ANL
Shipped Date: Sep 11, 2014
Inserted: Oct 08, 2014

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Sep 10, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	25	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	13	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	14	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	15	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	42	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	50	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	13	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	14	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	15	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	42	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	60	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	SaltRx	25	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	SaltRx	25	CrystalQuick standard profile, LBR round bottom plate
Oct 17, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	SaltRx	74	CrystalQuick standard profile, LBR round bottom plate
Mar 10, 2016	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	87	CrystalQuick standard profile, LBR round bottom plate