Purification

Target

IDP02160  

Purification ID

A18915  

Expression

M9 minimal (Sep 16, 2013)  

Experiment Date Start

Sep 26, 2013  

Experiment Date End

Sep 28, 2013  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

failed  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

178  

Final Volume (μL)

450  

Shipped To Lab Id

ANL  

Shipped Date

Oct 02, 2013  

Inserted

Jan 13, 2015