Purification

Target

IDP95363  

Purification ID

A19953  

Expression

LB (May 18, 2015)  

Experiment Date Start

Jan 10, 2015  

Experiment Date End

Jan 12, 2015  

Person

Boguslaw Nocek  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

PIG-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

25  

Final Volume (μL)

500  

Shipped To Lab Id

ANL  

Shipped Date

Jan 12, 2015  

Inserted

Jan 12, 2016