Purification

Target
IDP00259  
Purification ID
W1  
Expression
2xYT (Aug 03, 2009)  
Experiment Date Start
Dec 15, 2007  
Experiment Date End
Dec 16, 2007  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_inclusion_body_expression_2  
Status
success  
Description
Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.  
Affinity Tag Status
uncleaved  
SeMet Status
native  
Final Buffer
25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
10.5  
Final Volume (μL)
400  
Inserted
Oct 02, 2008