Purification

Target

IDP91145  

Purification ID

N1410  

Expression

Terrific Broth (Mar 23, 2016)  

Experiment Date Start

Mar 29, 2016  

Experiment Date End

Mar 31, 2016  

Person

Olga Kiryukhina  

Lab

Northwestern University  

Protocol

Autoinduction HTMC1-HTMC2  

Status

success  

Description

20 mM Ca2+ added to the lysis buffer, loading buffer and 5% imidazole.  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

 

Final Concentration (mg/mL)

15.8  

Final Volume (μL)

4600  

Shipped To Lab Id

 

Shipped Date

 

Inserted

Apr 02, 2016