Purification

Target
IDP63245  
Purification ID
A20493  
Expression
M9 minimal (Oct 15, 2014)  
Experiment Date Start
Oct 17, 2014  
Experiment Date End
Oct 19, 2014  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
1  
Final Volume (μL)
21600  
Shipped To Lab Id
ANL  
Shipped Date
Oct 19, 2014  
Inserted
Jun 15, 2016