Purification

Target
IDP63268  
Purification ID
A20551  
Expression
M9 minimal (May 21, 2016)  
Experiment Date Start
Sep 22, 2014  
Experiment Date End
Sep 24, 2014  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
1  
Final Volume (μL)
25000  
Shipped To Lab Id
ANL  
Shipped Date
Sep 24, 2014  
Inserted
Jun 22, 2016