Purification

Target

IDP07325  

Purification ID

A21026  

Expression

M9 minimal (Sep 23, 2016)  

Experiment Date Start

Oct 25, 2016  

Experiment Date End

Oct 27, 2016  

Person

Rory Mulligan  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

49  

Final Volume (μL)

550  

Shipped To Lab Id

ANL  

Shipped Date

Oct 27, 2016  

Inserted

Nov 04, 2016