Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP07222
Purification ID: A21043
Expression: M9 minimal (Sep 23, 2016)
Experiment Date Start: Nov 09, 2016
Experiment Date End: Nov 11, 2016
Person: Rory Mulligan
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 73
Final Volume (μL): 1200
Shipped To Lab Id: ANL
Shipped Date: Nov 11, 2016
Inserted: Nov 15, 2016

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Nov 10, 2016	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Emerald Biosystems/Wizard Full (I & II)	62
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	87	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	75	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	87	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	56	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	57	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	69	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	13	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Unspecified	26	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Natrix Screen	70	CrystalQuick standard profile, LBR round bottom plate
Nov 14, 2016	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	62	CrystalQuick standard profile, LBR round bottom plate
Nov 10, 2016	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Natrix Screen	84	CrystalQuick standard profile, LBR round bottom plate
Nov 14, 2016	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Natrix Screen	84	CrystalQuick standard profile, LBR round bottom plate