Purification

Target
IDP95481  
Purification ID
N1661  
Expression
Terrific Broth (May 03, 2016)  
Experiment Date Start
May 06, 2016  
Experiment Date End
May 07, 2016  
Person
Sergii Pshenychnyi  
Lab
Northwestern University  
Protocol
Autoinduction HTMC1-HTMC2  
Status
failed  
Description
As it has NusA-TVMV-6xHis-Strep-Tag® II-TEV-Protein complex structure, TVMV protease was applied to the protein lysate (O/N at +40C) first to release the 6xHis tag. After standard Affinity + Size Exclusion purification was performed. No pure fractions at proper MW found  
Affinity Tag Status
na  
SeMet Status
native  
Final Concentration (mg/mL)
0  
Final Volume (μL)
0  
Inserted
Feb 28, 2017