Purification

Target
CPX_96954_96956  
Purification ID
A22501  
Expression
M9 minimal (May 31, 2018)  
Experiment Date Start
Jun 25, 2018  
Experiment Date End
Jun 25, 2018  
Person
Lucy Stols  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cut  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
48  
Final Volume (μL)
500  
Shipped To Lab Id
ANL  
Shipped Date
Jun 27, 2018  
Inserted
Aug 20, 2018