Purification

Target

CPX_97279_97402  

Purification ID

A23319  

Expression

M9 minimal (Aug 12, 2019)  

Experiment Date Start

Aug 19, 2019  

Experiment Date End

Aug 21, 2019  

Person

Jessica Johnson  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

1  

Final Volume (μL)

10000  

Shipped To Lab Id

ANL  

Shipped Date

Aug 21, 2019  

Inserted

Nov 12, 2019