Purification

Target
CPX_97334_97364  
Purification ID
A23382  
Expression
LB + 40 mM K2HPO4 (Dec 11, 2019)  
Experiment Date Start
Jan 20, 2020  
Experiment Date End
Jan 23, 2020  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
23  
Final Volume (μL)
1500  
Shipped To Lab Id
ANL  
Shipped Date
Jan 22, 2020  
Inserted
Feb 25, 2020