Purification

Target

CPX_97405_97406  

Purification ID

A23517  

Expression

LB + 40 mM K2HPO4 (Apr 15, 2019)  

Experiment Date Start

Apr 17, 2019  

Experiment Date End

Apr 19, 2019  

Person

Robert Jedrzejczak  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

PIG-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

40  

Final Volume (μL)

1000  

Shipped To Lab Id

ANL  

Shipped Date

Apr 19, 2019  

Inserted

Sep 28, 2020