Purification

Target
IDP52003  
Purification ID
A23679  
Expression
LB + 40 mM K2HPO4 (Mar 09, 2021)  
Experiment Date Start
Mar 15, 2021  
Experiment Date End
Mar 17, 2021  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::SEC::SEC peak 1  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
0  
Final Volume (μL)
13500  
Shipped To Lab Id
ANL  
Shipped Date
Mar 17, 2021  
Inserted
Jul 12, 2021