Purification

Target

IDP01137  

Purification ID

A5040  

Expression

M9 minimal (Nov 12, 2008)  

Experiment Date Start

Jun 18, 2010  

Experiment Date End

Jun 20, 2010  

Person

Rory Mulligan  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

9  

Final Volume (μL)

1200  

Shipped To Lab Id

ANL  

Shipped Date

Jun 20, 2010  

Inserted

Jul 29, 2011