Purification

Target

IDP00141  

Purification ID

A2981  

Expression

M9 minimal + semet (Jun 21, 2010)  

Experiment Date Start

Jul 06, 2010  

Experiment Date End

Jul 08, 2010  

Person

Magdalena Makowska-Grzyska  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

10  

Final Volume (μL)

600  

Shipped To Lab Id

ANL  

Shipped Date

Jul 08, 2010  

Inserted

Jul 29, 2011