Purification

Target

IDP02020  

Purification ID

A4604  

Expression

M9 minimal + semet (Mar 10, 2009)  

Experiment Date Start

Mar 23, 2009  

Experiment Date End

Mar 25, 2009  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleavage unsuccessful  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

11  

Final Volume (μL)

250  

Shipped To Lab Id

ANL  

Shipped Date

Mar 25, 2009  

Inserted

Jul 29, 2011