Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00629
Purification ID: A2811
Expression: M9 minimal (Jun 08, 2010)
Experiment Date Start: Jun 18, 2010
Experiment Date End: Jun 21, 2010
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 33
Final Volume (μL): 1100
Shipped To Lab Id: ANL
Shipped Date: Jun 21, 2010
Inserted: Jul 29, 2011

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	11	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	2	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	44	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-1	21	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	19	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	37	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	90	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	49	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	53	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	6	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	49	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	72	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	81	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	29	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	87	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	2	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	11	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	13	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	79	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	43	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	72	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	25	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	93	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	47	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	31	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	50	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	51	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-1	20	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-1	22	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-1	85	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-1	95	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	52	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	80	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	18	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	6	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	72	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	40	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	75	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	21	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	28	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	31	CrystalQuick standard profile, LBR round bottom plate
Jun 23, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	80	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	1	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	8	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	19	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	20	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	88	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	91	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	93	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	43	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	46	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	14	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	18	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	15	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	16	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	31	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	45	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	89	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	80	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	18	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	29	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	80	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	4	CrystalQuick standard profile, LBR round bottom plate
Jul 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	87	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	6	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	72	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	73	CrystalQuick standard profile, LBR round bottom plate
Jul 22, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	The PEGsII Suite	7	CrystalQuick standard profile, LBR round bottom plate