Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP01178
Purification ID: A2638
Expression: M9 minimal (Oct 26, 2010)
Experiment Date Start: Nov 01, 2010
Experiment Date End: Nov 02, 2010
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 35
Final Volume (μL): 1900
Shipped To Lab Id: ANL
Shipped Date: Nov 02, 2010
Inserted: Jul 29, 2011

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	32	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	41	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	79	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	52	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	56	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	46	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	52	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	56	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	56	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	30	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	52	CrystalQuick standard profile, LBR round bottom plate
Nov 02, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	15	CrystalQuick standard profile, LBR round bottom plate