Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP05539
Purification ID: A6110
Expression: M9 minimal + semet (May 24, 2011)
Experiment Date Start: Jun 03, 2011
Experiment Date End: Jun 06, 2011
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: semet
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 36
Final Volume (μL): 500
Shipped To Lab Id: ANL
Shipped Date: Jun 06, 2011
Inserted: Jul 29, 2011

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	40	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	89	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	40	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	45	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	89	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-1	54	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	63	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	14	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	34	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	58	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	94	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	11	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	14	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	34	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	70	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	94	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	15	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	61	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	63	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	66	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	61	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	63	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	66	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	11	CrystalQuick standard profile, LBR round bottom plate
Jun 07, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	45	CrystalQuick standard profile, LBR round bottom plate