Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP90677
Purification ID: A3062
Expression: 2xYT (Nov 09, 2010)
Experiment Date Start: Nov 15, 2010
Experiment Date End: Nov 17, 2010
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 69
Final Volume (μL): 900
Shipped To Lab Id: ANL
Shipped Date: Nov 17, 2010
Inserted: Jul 29, 2011

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Apr 20, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	91	CrystalQuick standard profile, LBR round bottom plate
Dec 06, 2011	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	91	CrystalQuick standard profile, LBR round bottom plate
Nov 18, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	38	CrystalQuick standard profile, LBR round bottom plate
Nov 18, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	45	CrystalQuick standard profile, LBR round bottom plate
Nov 18, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	43	CrystalQuick standard profile, LBR round bottom plate
Nov 18, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	46	CrystalQuick standard profile, LBR round bottom plate